GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE FROM RABBIT MUSCLE
作者： R.M. Scheek , E.C. Slater
DOI: 10.1016/S0076-6879(82)89055-1
关键词:
摘要: Publisher Summary The rabbit muscle is one of the most frequently used sources for glyceraldehyde-3-phosphate dehydrogenase. This chapter describes assay method and properties enzyme, dehydrogenase, isolated from muscle. Classical isolation procedures involve high solubility nicotinamide adenine dinucleotide kinase (NAD + )-containing enzyme in (NH 4 ) 2 SO solutions; several recrystallization steps concentrated solutions at pH values above 8.0 are also involved. involved purification procedure (1) extraction muscle, (2) ammonium sulfate fractionation, (3) chromatography. preparation holoenzyme, apoenzyme, tetra (3-phosphoglyceroyl) discussed chapter. specific activity prepared about 185 units per milligram protein. migrates as a single band polyacrylamide gel electrophoresis has molecular weight 145,000. It contains 3.8–4.0 highly reactive cysteine residues, determined with dithionitrobenzoate, can bind 4.0 molecules NAD or dehydrogenase (NADH) molecule.
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