Efficient production of membrane‐integrated and detergent‐soluble G protein‐coupled receptors in Escherichia coli

摘要: G protein-coupled receptors (GPCRs) are notoriously difficult to express, particularly in microbial systems. Using GPCR fusions with the green fluorescent protein (GFP), we conducted studies identify bacterial host effector genes that result a general and significant enhancement amount of membrane-integrated human GPCRs can be produced Escherichia coli. We show coexpression membrane-bound AAA+ protease FtsH greatly enhances expression yield four different class I GPCRs, irrespective presence GFP. this new system, 0.5 2 mg/L detergent-solubilized purified full-length central cannabinoid receptor (CB1) bradykinin (BR2) shake flask cultures, respectively, two proteins had previously eluded