Use of FISH to detect chromosomal translocations and deletions. Analysis of chromosome rearrangement in synovial sarcoma cells from paraffin-embedded specimens.

摘要: Retrospective cytogenetic analysis was performed on paraffin-embedded cells from five cases of synovial sarcoma to evaluate the frequency X;18 translocation characteristic this tumor. Fluorescent in situ hybridization with DNA probes for centromeres chromosomes X and 18 used whole chromosome painting 18. Translocation inferred when there were only two centromere signals but three probe unequal size. On basis it possible identify t(X;18) cases. The fourth case found have extra copies without translocation, while fifth case, one a questionable diagnosis, had normal pattern minor clone showing translocated X. Thus study demonstrates feasibility value using fluorescent detect rearrangements archival tumor specimens.