Ligand effects on the binding of cis- and trans-[PtCl(2)Am(1)Am(2)] to proteins.

摘要: As part of a systematic study the basic principles that govern formation and reactivity Pt–protein adducts, we report effect substituting ammine ligand cis- trans-[PtCl2(NH3)2] complexes with bulkier planar aromatic or nonplanar cyclic amine ligands on binding properties to ubiquitin horse heart myoglobin. The replacement had different cis trans isomers investigated. In cis-Pt complexes, replacing one both by piperidine 4-picoline dramatically decreased proteins studied, whereas in substituted trans-Pt increased rate. This behavior may have do preferred sites complexes. investigated also did not display preference for Met1 ubiquitin, possibly owing steric constraints imposed ligands. introduction charged piperazine significantly rate protein, electrostatic interactions hydrogen-bond formations surface protein. myoglobin does disrupt folding as judged electrospray ionization mass spectrometry.