Screening and reaction engineering for the bioreduction of ethyl benzoylacetate and its analogues

摘要: Abstract Microbial reduction of benzoylacetates is already an established part the synthetic toolbox to obtain chiral ethyl 3-hydroxy-3-phenylpropionate although bioreduction yields are low moderate. A 30% increase in enantioselectivity 99% ee and a significant improvement around 85% were achieved by combining simple screening procedures reaction engineering strategy. Three experimental parameters selected for investigation: influences glucose, enzymatic inhibitor biocatalyst immobilization. The screened yeasts Pichia kluyveri , stipitis Candida utilis found give better ee's benzoylacetate 1a p -nitrobenzoylacetate 1b -methoxybenzoylacetate 1c respectively, with addition α-chloroacetophenone as immobilization alginate beads. Our results demonstrate that optimized process can be implemented on preparative scale without any loss yield ee.