In vitro antiviral activity of circular triple helix forming oligonucleotide RNA towards Feline Infectious Peritonitis virus replication.
作者： Oi Kuan Choong , Parvaneh Mehrbod , Bimo Ario Tejo , Abdul Rahman Omar
DOI: 10.1155/2014/654712
关键词:
摘要: Feline Infectious Peritonitis (FIP) is a severe fatal immune-augmented disease in cat population. It caused by FIP virus (FIPV), virulent mutant strain of Enteric Coronavirus (FECV). Current treatments and prophylactics are not effective. The vitro antiviral properties five circular Triple-Helix Forming Oligonucleotide (TFO) RNAs (TFO1 to TFO5), which target the different regions feline coronavirus (FCoV) FIPV WSU 79-1146 genome, were tested FIPV-infected Crandell-Rees Kidney (CRFK) cells. RT-qPCR results showed that TFO RNAs, except TFO2, inhibit replication, where viral genome copy numbers decreased significantly 5-fold log10 from 1014 virus-inoculated cells 109 RNAs-transfected Furthermore, binding RNA with targeted segment was also confirmed using electrophoretic mobility shift assay. strength kinetics between their demonstrated NanoITC In conclusion, TFOs have potential be further developed as agents against infection.
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