Inhibitory effects of ribavirin alone or combined with human alpha interferon on feline infectious peritonitis virus replication in vitro.

摘要: Abstract The antiviral activities of ribavirin (1-β- D -ribofuranosyl-1,2,4-triazole-3-carboxamide; virazole), either alone or in combination with recombinant human leukocyte (alpha) interferon (rHuIFN-α), were evaluated against feline infectious peritonitis virus (FIPV) kidney-cell cultures. 50% inhibitory dose (ID 50 ) for uninfected, rapidly dividing cells was ∼17 μ g ml −1 whereas the ID FIPV 2.5 . therapeutic index (TI) (i.e. ratio minimum cell-toxic to virus-inhibitory dose) 6.8. Although a dose-dependent inhibition viral infectivity occurred at non-toxic doses, maximum effects (⩾4 log 10 reduction FIPV) cytotoxic doses. When low moderate doses combined 100 U rHuIFN-α , resulting significantly greater than sum observed from alone. Significant synergistic interactions 1, 5, 12.5 and 25 Synergistic combinations produced up an 80-fold 200-fold relative increase compared that by equivalent respectively, In cell growth studies, addition test did not anticellular effect alone; seemingly, potentiation activity independent any additive effects. Potentially, two agents vivo may decrease required thus reduce drug toxicity.