Chromium(VI) Induces p53-Dependent Apoptosis in Diploid Human Lung and Mouse Dermal Fibroblasts

摘要: Some forms of hexavalent chromium [Cr(VI)] are known to cause damage respiratory-tract tissue and DNA thought be human lung carcinogens. In general, Cr(VI) is mutagenic carcinogenic at doses that also evoke some cell death, we previously showed the predominant mode death apoptosis. Because p53 has been shown initiate apoptosis after genotoxic insults, objective these experiments was determine whether activated in necessary for normal diploid fibroblasts (HLF cells) exposure. By using annexin(V) staining fluorescent microscopy, found caused up 14% HLF cells undergo within 24 h addition, by western blotting, protein levels increased fourfold sixfold exposure sodium chromate. major function as a transcription factor, it must translocated from cytoplasm nucleus chromate active. Immunofluorescence studies an antibody against that, exposure, located treated cells. The necessity chromium-induced examined two ways. One approach used dermal wild-type, heterozygous, null mice, other were transiently transfected with papilloma virus E6 gene, which targets degradation creates functional p53-null cell. These dependent. Mol. Carcinog. 28:111-118, 2000.