Non-enzymatic phosphorylation of bovine serum albumin by Cr(V) complexes: role in Cr(VI)-induced phosphorylation and toxicity.

摘要: Evidence for the non-enzymatic phosphorylation of bovine serum albumin (BSA) by sodium bis(2-ethyl-2-hydroxybutyrato)oxochromate(V), Na[CrVO(ehba)2], 1, bis(2-hydroxy-2-methylbutyrato)oxochromate(V), Na[CrVO(hmba)2], 2 and potassium dichromate, K2Cr2O7, 3 in presence labeled adenosine-5′-triphosphate (ATP) under conditions physiological pH is presented. Aggregation extent BSA mediated or seems to increase with concentration time incubation reaction mixture containing all reactants. The [γ-32P] label ATP incorporated into aggregates vitro protein 3. Phosphorylation absence negligible. Addition EDTA reverses aggregation liberates partially phosphate label. stoichiometry found be highest equal 12.25 mol PO43−/mol 500 μM which decreases 10.56 after treatment. Resistance removal increases incubation. Dialysis phosphorylated only partially, indicating formation covalent links groups BSA. site being hydroxyl side tyrosine serine/threonine residues has been gained. Based on results, a possibility that mimic function kinases invoked.