STABILITY OF AMPLIFIED DNA IN CHINESE HAMSTER OVARY CELLS
作者： N.H. Cossons , P.M. Hayter , M.F. Tuite , N. Jenkins
DOI: 10.1016/B978-0-7506-1103-9.50063-9
关键词:
摘要: ABSTRACT A Chinese Hamster Ovary (CHO) cell line containing the human gamma interferon (IFN-∝) gene co-amplified with for dihydrofolate reductase (DHFR), was continuously maintained 120 days in serum-free medium 0.1 μ M methotrexate. During this time IFN-∝ titre fell by 70% due to a reduction specific IFN production rate. Cell number higher late passage cultures and viability longer periods. Chromosome studies revealed no significant difference overall chromosome frequency distribution, except 16% increase modal of 18. Southern blot analysis DHFR showed 45% decrease levels over decreasing 30% same period. Keywords : CHO, stability, amplification, DHFR, interferon,
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