Stability of protein production from recombinant mammalian cells.

摘要: One of the most important criteria for successful generation a therapeutic protein from recombinant cell is to obtain line that maintains stability production. If this not achieved it can generate problems process yields, effective use time and money, regulatory approval products. However, selection sustains production over required period may be difficult achieve during development protein. There are several studies in literature have reported on instability lines. The causes varied and, many cases, exact molecular mechanisms unknown. proteins by cells modulated events at levels ranging transcription, posttranscriptional processing, translation, posttranslational secretion. potential regulation or all these stages. In study we review published information three industrially lines: hybridoma, Chinese hamster ovary (CHO), nonsecreting (NS0) myeloma We highlight likely loci which engendered indicate other areas affect mammalian cells. also outline approaches could help overcome associated with unpredictable expression maximized production, consequences might