Neurospora 6-Phosphogluconate Dehydrogenase II. PROPERTIES OF TWO PURIFIED MUTANT ENZYMES

摘要: Abstract Mutations in two unlinked genes of Neurospora crassa, col-10 and col-3, lead to morphological abnormalities variant 6-phosphogluconate dehydrogenases (EC 1.1.1.44). The purified mutant enzymes have specific activities, 30 units per mg, similar the wild type dehydrogenase, but differ kinetic parameters, isoelectric points, thermolability. These properties heterocaryon are intermediate between those enzymes. Since morphology mutants is restored nearly that heterocaryons, there a correlation severity dehydrogenase defects. results suggest col-3 control structure altered responsible for morphologies.