High Performance Liquid Chromatographic Assay for Adinazolam Mesylate in Rodent Feed Mixtures

摘要: Abstract Adinazolam mesylate was recovered from the feed mixtures by repeated extraction with a pH 4 citrate buffer. The pooled extracts were diluted water when necessary and mixed an equal volume of acetonitrile solution internal standard (naphthalene). After mixing centrifugation, clear supernates separated for chromatography. chromatography carried out on reverse phase column using mixture phosphate buffer, methanol in ratios 85:40:20 as mobile phase. eluted columns at about 11.7–12 15.50–17.5 minutes, respectively. peak height adinazolam concentrations showed excellent linearity (r>0.999). Extracts blank samples did not show interference to assay. Assay results satisfactory accuracy precision obtained. methodology applicable assay drug-feed containing 0.02 10.0 mg per...