Effects of ovarian steroids and raloxifene on proteins that synthesize, transport, and degrade serotonin in the raphe region of macaques.

摘要: In the monkey dorsal raphe, we reported that 1-month (mo) of estrogen replacement, with or without progesterone supplementation for 14 days, significantly increased tryptophan hydroxylase-1 (TPH-1) mRNA; decreased serotonin reuptake transporter (SERT) mRNA and monoamine oxidase (MAO)-A mRNA, but had no effect on MAO-B mRNA. Here, questioned what would 1 5 mo ovarian hormones selective receptor modulator (SERM), raloxifene, have TPH protein phosphorylation, expression SERT, MAO-A MAO-B? Raloxifene antagonizes in breast uterus, estrogen-like activities brain been reported. Cytoplasmic membrane extracts raphe region were processed Western blotting. TPH, phosphoserine, MAO-A, detected specific antibodies. The optical densities signals measured NIH image analyzed by ANOVA. Both estrogen, progesterone, raloxifene protein. Administration plus also phosphorylation. Estrogen, SERT However, Estrogen alone combined caused a significant reduction MAO-A. Yet, after same treatments, was not different from spayed controls. MAO-B. addition Thus, to various extents, may increase production transport. degradative enzymes suggests complex combination gene transcription, post-transcriptional processing, substrate feedback mechanisms.