Tear fluid biomarker profiling: a review of multiplex bead analysis.
作者： Suzanne Hagan , Alan Tomlinson
DOI: 10.1016/J.JTOS.2013.04.004
关键词:
摘要: Developments in the field of tear film protein profiling are reviewed, including advantages and disadvantages multiplex bead array technique its potential for identifying biomarkers ocular surface disease. Commercial kits, which specifically employ 96-well plates with polystyrene microspheres magnetic beads, sensitivity variations between them discussed. Modifications to protocols these kits (which designed primarily larger sample volumes, such as blood cell culture supernatants) may be necessary fluid samples. Multiplex is compared "gold standard," ELISA. The challenges diagnosing monitoring dry eye disease, due conflicting variable signs symptoms presented, illustrated by authors' own study results. development a biomarker profile disease would useful approach ongoing problems diagnosis. has important applications this regard.
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