Formation of divalent ion mediated anionic disc bicelle-DNA complexes.

摘要: Disc-shaped bicelles are formed by mixing long-chain lipids with short-chain at suitable molar ratios and they have a relatively uniform size, typically around few tens of nanometers in diameter. Different from the formulated cationic or anionic liposome–DNA complexes, which used as nonviral vectors for improving transfection efficiency gene therapy, novel way packing DNA can be developed using much smaller disc-like bicelles. We demonstrate that lipid bicelle-ion–DNA (AB–DNA) complexes help divalent ions. Multi-stacked AB–DNA diameters 50–100 nm lengths 50–150 revealed TEM. Using lipid–DNA has advantage lower cytotoxicity than lipids. The interaction was investigated SAXS. It found bicelle could not form stable low calcium ion concentrations, such 1 mM. range 10 mM to 100 concentrations. However, an equal charge system, ion-membrane phase (multilamellar vesicles) would gradually appear concentration is increased above critical concentration. indicates packed closer If more added two-phase coexistence system (originally total DNA), transformed into complexes. As result,