Investigation of Binary Lipid Mixtures of a Three-Chain Cationic Lipid with Phospholipids Suitable for Gene Delivery.
作者： Christian Wölk , Christopher Janich , Annette Meister , Simon Drescher , Andreas Langner
DOI: 10.1021/ACS.BIOCONJCHEM.5B00505
关键词:
摘要: In the present work, we characterize binary lipid mixtures consisting of a three-chain amino-functionalized cationic (DiTT4) with different phospholipids, namely, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPE), or 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC). The mixing behavior was investigated by differential scanning calorimetry (DSC). Additionally, aqueous dispersions were characterized means dynamic light scattering (DLS), laser Doppler electrophoresis, and transmission electron microscopy (TEM) to get further information about particle size, charge, shape. complex formation between plasmid DNA (pDNA) zeta-(ζ)-potential (laser electrophoresis) DLS measurements, lipid/DNA complexes (lipoplexes) screened for efficient transfer (transfection) in cell culture. Finally, compositions respect serum stability. This work provides detailed characterization as foundation research. Efficient gene presence demonstrated selected lipoplexes showing their capability be used high-potency delivery vehicles.
acs.org
mpg.de
core.ac.uk
sci-hub.se 参考文章(50)
Ayesha Ahmad, Heather M. Evans, Kai Ewert, Cyril X. George, Charles E. Samuel, Cyrus R. Safinya, New multivalent cationic lipids reveal bell curve for transfection efficiency versus membrane charge density: lipid-DNA complexes for gene delivery The Journal of Gene Medicine. ,vol. 7, pp. 739- 748 ,(2005) , 10.1002/JGM.717
Po-Wei Yang, Tsang-Lang Lin, Yuan Hu, U-Ser Jeng, Formation of divalent ion mediated anionic disc bicelle-DNA complexes. Soft Matter. ,vol. 10, pp. 2313- 2319 ,(2014) , 10.1039/C3SM52775A
Ming-Kung Yeh, Hsin-I Chang, Ming-Yen Cheng, Clinical development of liposome-based drugs: formulation, characterization, and therapeutic efficacy. International Journal of Nanomedicine. ,vol. 7, pp. 49- 60 ,(2011) , 10.2147/IJN.S26766
Reto A. Schwendener, Liposomes as vaccine delivery systems: a review of the recent advances Therapeutic Advances in Vaccines. ,vol. 2, pp. 159- 182 ,(2014) , 10.1177/2051013614541440
F Szoka, D Papahadjopoulos, Comparative Properties and Methods of Preparation of Lipid Vesicles (Liposomes) Annual Review of Biophysics and Bioengineering. ,vol. 9, pp. 467- 508 ,(1980) , 10.1146/ANNUREV.BB.09.060180.002343
Inge S. Zuhorn, Udo Bakowsky, Evgeny Polushkin, Willy H. Visser, Marc C.A. Stuart, Jan B.F.N. Engberts, Dick Hoekstra, Nonbilayer phase of lipoplex-membrane mixture determines endosomal escape of genetic cargo and transfection efficiency. Molecular Therapy. ,vol. 11, pp. 801- 810 ,(2005) , 10.1016/J.YMTHE.2004.12.018
Po-Wei Yang, Tsang-Lang Lin, Tsung-Yi Lin, Ching-Hsun Yang, Yuan Hu, U-Ser Jeng, Packing DNA with disc-shaped bicelles Soft Matter. ,vol. 9, pp. 11542- 11548 ,(2013) , 10.1039/C3SM51703A
Peggy Scholtysek, Anja Achilles, Claudia-Viktoria Hoffmann, Bob-Dan Lechner, Annette Meister, Carsten Tschierske, Kay Saalwächter, Katarina Edwards, Alfred Blume, A T-shaped amphiphilic molecule forms closed vesicles in water and bicelles in mixtures with a membrane lipid. Journal of Physical Chemistry B. ,vol. 116, pp. 4871- 4878 ,(2012) , 10.1021/JP207996R
Lucyanna Barbosa-Barros, Gelen Rodríguez, Clara Barba, Mercedes Cócera, Laia Rubio, Joan Estelrich, Carmen López-Iglesias, Alfonso de la Maza, Olga López, Bicelles: Lipid Nanostructured Platforms with Potential Dermal Applications Small. ,vol. 8, pp. 807- 818 ,(2012) , 10.1002/SMLL.201101545
Christian Wölk, Christopher Janich, Dorota Pawlowska, Simon Drescher, Annette Meister, Gerd Hause, Bodo Dobner, Andreas Langner, Gerald Brezesinski, Lamellar versus micellar structures-aggregation behavior of a three-chain cationic lipid designed for nonviral polynucleotide transfer ChemPhysChem. ,vol. 16, pp. 2115- 2126 ,(2015) , 10.1002/CPHC.201500188