Priming of Mesenchymal Stem Cells with Oxytocin Enhances the Cardiac Repair in Ischemia/Reperfusion Injury

摘要: Oxytocin stimulates the cardiomyogenesis of embryonic stem cells and adult cardiac cells. We previously reported that oxytocin has a promigratory effect on umbilical cord blood-derived mesenchymal (UCB-MSCs). In this study, UCB-MSCs were cultured with examined for their therapeutic in an infarcted heart. pretreated 100 nM markers assessed by immunofluorescence staining. Next, oxytocin-supplemented USC-MSCs (OT-USCs) cocultured hypoxia/reoxygenated neonatal rat cardiomyocytes dye transfer then examined. For vivo ischemia/reperfusion was induced rats, phosphate-buffered saline (group 1), 1-day OT-USCs 2), or 7-day 3) injected into myocardium. Two weeks after injection, histological changes function expressed connexin 43 (Cnx43), troponin I (cTnI), α-sarcomeric actin (α-SA) supplementation coculture cardiomyocytes. Functional gap junction formation greater group 3 than groups 1 2. Cardiac fibrosis macrophage infiltration lower Restoration Cnx43 expression Cnx43- cTnI-positive peri-infarct zone observed 2 more frequently 3. The ejection fraction (EF) increased weeks. improved EF sustained 4 only Our findings suggest can contribute to cardiogenic potential repair.