Regulation of IFN-λ1 Promoter Activity (IFN-λ1/IL-29) in Human Airway Epithelial Cells
作者： Rachael Siegel , Joyce Eskdale , Grant Gallagher
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摘要: The type III (λ) IFNs (IFN-λ1, IFN-λ2, and IFN-λ3) their receptor are the most recently discovered IFN family. They induced by viruses mediate antiviral activity, but have an important, specific functional niche at immune/epithelial interface, as well in regulation of Th2 cytokines. Their expression appears diminished bronchial epithelial cells rhinovirus-infected asthmatic individuals. We investigated IFN-λ1 human airway using reporter genes analysis, chromatin immunoprecipitation, small interfering RNA knockdown, DNase footprinting. In this article, we define c-REL/p65 NF-κB heterodimer IRF-1 key transcriptional activators ZEB1, B lymphocyte-induced maturation protein 1, p50 homodimer repressors gene. further show that ZEB1 selectively regulates IFNs. To our knowledge, study presents first characterization any promoter its native context conformation can now be applied to understanding pathogenic dysregulation disease.
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