Multiscale Mechanical Evaluation of Human Supraspinatus Tendon Under Shear Loading After Glycosaminoglycan Reduction.

摘要: Proteoglycans (PGs) are broadly distributed within many soft tissues and, among other roles, often contribute to mechanical properties. Although PGs, consisting of a core protein and glycosaminoglycan (GAG) sidechains, were once hypothesized regulate stress/strain transfer between collagen fibrils help support load in tendon, several studies have reported no changes tensile mechanics after GAG depletion. Since GAGs known sustain nontensile loading tissues, we that might shear human supraspinatus tendon (SST), commonly injured which functions complex multiaxial environment. Therefore, the objective this study was determine whether response SST shear, specifically terms multiscale properties mechanisms microscale matrix deformation. Results showed chondroitinase ABC (ChABC) treatment digested while not disrupting fibers. Peak equilibrium stresses decreased only slightly ChABC significantly different from pretreatment values. Reduced stress ratios computed shown be greater compared phosphate-buffered saline (PBS) incubation without enzyme, suggesting these relatively small values due strictly tissue swelling. Microscale deformations also treatment. This demonstrates possibly play minor role contributing behavior but key constituent mechanics.