Microarray Technology in Practice

摘要: Using chips composed of thousands spots, each with the capability holding DNA molecules corresponding to a given gene, microarray technology has enabled researchers measure simultaneously gene expression across genome. As other large-scale genomics approaches, technologies are broadly applicable disciplines life and biomedical sciences, but remain daunting many researchers. This guide is designed demystify inform more biologists about this critically important experimental technique. * Cohesive overview available platforms, followed by detailed discussion design analysis experiments Up-to-date description normalization methods current for sample amplification labeling Deep focus on oligonucleotide design, printing, hybridization, data acquisition, normalization, meta-analysis Additional uses such as ChIP (chromatin immunoprecipitation) hybridization arrays, microarray-based comparative genomic (CGH), cell tissue arrays