Development of a Novel DNA Microchip for Pathogen Detection

摘要: Although DNA microarray can detect multiple samples simultaneously, current detection techniques involve PCR and other traditional procedures. In this study, a sensitive, specific rapid method, which eliminates lengthy processes, for pathogenic is presented. This technology based on the hybridization of target to immobilized probe, extension probe DNAs using target-DNA as template signal generation by streptavidin-horseradish peroxidase substrate. method highly allowing single-nucleotide-base mismatches discrimination at femtomole level. The experiments are designed achieve short time. Therefore, satisfactory be detected within minutes, DNA. Most importantly, E. coli genomic technology. conclusion, useful applications including on-site disease detection, crime scene investigation, pathogen inspection in environment. INDEX WORDS: microchip, microarray, chemiluminescent Pathogenic Genomic detection. DEVELOPMENT OF A NOVEL MICROCHIP FOR PATHOGEN DETECTION