A Beginner's Guide to Microarrays

摘要: 1: Slide Coating And DNA Immobilization Chemistries K. Aboytes, J. Humphreys, S. Reis, B. Ward. Introduction. Glass Properties. Cleaning. Chemistries. Surface Analysis Methods. Summary. References. 2: Diagnostic Oligonucleotide Microarrays For Microbiology L. Bodrossy. Scheme Of The Experimental Approach. Sources Variation. Establishment A Sequence Database. Oligo Length Melting Temperature (TM) Designing Sets Tuned To Work Together. Set Design. Choice Oligo/Surface Binding Chemistry. Array Printing. Target Preparation. Hybridisation. Scanning. Data Analysis. Applications In Microbial Identification. WWW Sites Related Microarrays. AS Biosensors. Reference List. 3: Printing Technologies Microarray Manufacturing Techniques: Making Perfect T. Martinsky. Manufacturing. Comparing Technologies. Conclusions. Acknowledgments. 4: Arrays Masses - Setting Up Core Facility R.P. Searles. Preface. Hedco/Oregon Cancer Institute Spotted At OHSU. Set-Up. Printer. Slides. Amplification. Array. Hybridization. Scanner. Other Equipment. Conclusion. 5: Normalization: Art Science Overcoming Technical Variance Maximize Detection Biologic M.A. Sartor, M. Medvedovic, B.J. Aronow. Correcting Order Study Biological Single Channel Normalizations. Normalizations Two-Channel Data. Role Design Removal Variance. Gene-Specific Clustering. 6: E. Blalock. Measuring RNA. Fold Change Significance. Fold-Change. Affymetrix Working With More Than Two Groups. Functional Grouping. Using Excel. Acknowledgements. 7: Experiment Statistical Xuejun Peng, A.J. Stromberg. Experiment. General Procedures Multiple Hypothesis Testing Experiments. Methods Based On P Value Adjustment. Summary Chapter. Some Useful Online 8: Strategies Clustering, Classifying, Integrating, Standardizing Visualizing Gene Expression W. Valdivia Granda. Matrix. Distance Functions. Unsupervised Clustering Validating Supervised Nearest Neighbors. Support Vector Machines. Improve Classifier Performance. Genetic Biochemical Networks. Additional Microarray. Visualization. Standardization Integration. Markup Language (MAGE-ML). Repositories. Challenges