Differential expression of the complement regulatory proteins in the human eye.

摘要: PURPOSE: The presence of complement activation products in the human eye during infection or inflammation has been well described. During host must be protected from attack against self tissue; this is achieved by three membrane-bound regulatory proteins: membrane cofactor protein (MCP, CD46), decay accelerating factor (DAF, CD55), and complex inhibiting (CD59). This study was undertaken to analyze expression these proteins normal eye. METHODS: Tissues were sectioned cryostat both polyclonal monoclonal antibodies MCP, DAF, CD59 used. Control stains performed with nonrelevant same immunoglobulin subclass rabbit serum as omission primary secondary antibodies. RESULTS: All found differentially expressed With anti-MCP, strong staining corneal epithelium weak keratocytes stroma photoreceptor cells observed. Staining anti-DAF very ciliary body moderate (keratocytes) iris. In contrast, anti-CD59 stained strongly epithelium, (keratocytes), iris, choroid, all layers retina, moderately body. CONCLUSIONS: Identification provides evidence that a system exists protect destruction complement-activating events. It remains determined if other more specialized functions exist for proteins, especially case because its extensive retina.