Elevated expression of IL2 is associated with increased infiltration of CD8+ T cells in biliary atresia

摘要: Abstract Aim of Study Lymphocyte-mediated inflammation within the liver and bile ducts has been proposed as a potential mechanism in pathogenesis biliary atresia (BA). Recent reports have suggested that BA is associated with upregulation T H 1-type inflammatory cytokines. Quantitative real-time polymerase chain reaction (qrt-PCR) useful tool to assess immune cell density cytokine gene expression tissues. The aim this study was characterize by quantifying lymphocyte 1 cytokines such IL2 tumor necrosis factor α (TNF- ) biopsies using qrt-PCR. Methods Total RNA extracted from archival formalin-fixed paraffin-embedded biopsy specimens collected three age-matched groups patients: group I, nonsyndromic at time Kasai portoenterostomy (n = 10); II, choledochal dilatation surgery 2); III, other cholestatic diseases including hypoplasia parenteral nutrition–associated cholestasis 3). Qrt reverse transcription–PCR performed measure relative levels messenger (mRNA) CD4, CD8, IL2, TNF- . Their were normalized level β -actin expression. numbers CD4 + CD8 lymphocytes infiltrating portal tracts also measured immunohistochemistry. Data expressed median ranges, statistical comparison correlation nonparametric test (Kruskal-Wallis Spearman rank). Results mRNA significantly increased I (median 0.26, range 0.11-6.29) compared II III (0.002, 0-0.004 0.02, 0-0.12) ( P III. There significant between number Conclusion Increased T-cell infiltration infants suggest possibility plays role condition.