Sub1 and Maf1, Two Effectors of RNA Polymerase III, Are Involved in the Yeast Quiescence Cycle

摘要: Abstract Sub1 and Maf1 exert an opposite effect on RNA polymerase III transcriptioninterfering with different steps of the transcription cycle. In this study, we presentevidence that also exhibit role yeast chronologicallife span. First, cells lacking need more time than wild type to exit from restingand lag in re-proliferation is correlated a delay transcriptionalreactivation. Second, our data show capacity properlyestablish quiescent state impaired absence resulting apremature death dependent Ras/PKA Tor1/Sch9 signallingpathways. On other hand, maf1D are long-lived mutantsuggesting connection between Pol longevity. Introduction its natural environment, budding Saccharomyces cerevisiae spendsmost life where it can maintain both viability thecapacity re-enter proliferation cycle upon addition carbon source.The length non-dividing population maintains definesits chronological span (CLS). For years now, CLS provides fruitfulmodel for aging research many progress have been made understand themechanisms regulating cell quiescence cycle, including entry intoquiescence, maintenance re-proliferate. Anumber dramatic morphological, physiological biochemical changes [1,2]