In silico development of simple sequence repeat markers within the aeschynomenoid/dalbergoid and genistoid clades of the Leguminosae family and their transferability to Arachis hypogaea, groundnut

摘要: Cultivated groundnut (Arachis hypogaea L.) is an agronomically and economically important oilseed crop grown extensively throughout the semi-arid tropics of Asia, Africa Latin America. The genetic base cultivated very narrow as a result bottleneck associated with recent polyploidization which makes it critical to determine levels diversity within available germplasm collections prior breeding. In groundnut, use SSRs for assessment may offer potential reveal variation genome species. An alternative bioinformatics, or in silico approach, identifying suitable application presented, low-cost wet lab SSR identification. All nucleotide sequences from species aeschynomenoid/dalbergoid genistoid clades Leguminosae family were searched motifs primers designed 109 unique SSRs. Representative accessions six genera selected assessing SSR-transferability rates. total, 60% total cross-genera transfer testing reactions gave prominent reproducible amplicons, 51 amplifying A. hypogaea. These further tested against 27 diverse Arachis 18 revealed polymorphism, demonstrating that approach identification development valid strategy lesser-studied crops.