Application of Next Generation Sequencing and Genotyping Technologies to Develop Large‐Scale Genomic Resources in SAT Legume Crops

摘要: Molecular markers and genetic maps are the pre‐requisites for trait mapping genomics‐assisted crop improvement. However, very limited genomic resources were available until recently legume crops important in semi‐arid tropics (SAT). As a part of several initiatives, species‐specific now being developed in most these crops. For instance, using simple sequence repeat (SSR)‐enriched libraries and bacterial artificial chromosome (BAC)‐end mining approaches, nearly 1,500‐ 3,000 novel SSR markers have been developed chickpea, pigeonpea groundnut. In addition, next generation sequencing technologies like Roche 454/FLX Illumina/Solexa, addition to Sanger sequencing, being used the transcriptomes reference or parental genotypes populations chickpea access the gene space develop functional markers. Based on transcript reads, transcriptome assemblies have (103,215 tentative unique sequences, TUSs) (127,754 TUSs) that characterized genome data Medicago soybean. parallel, RNA four chickpea twelve genotypes, represent parents different populations, been sequenced by Illumina/Solexa approach has resulted ca. 120 million reads 20 million pigeonpea. Alignment with transcriptome assembly respective species provided large number (tens thousands) SNPs. Selected set SNPs are being large‐scale SNP genotyping platform By existing resource SSR, DArT markers, high‐density trait mapping molecular breeding. It is anticipated breeding practice may be routine of breeding activities SAT legumes coming future