INSECT: IN-silico SEarch for Co-occurring Transcription factors

摘要: Motivation: Transcriptional regulation occurs through the concerted actions of multiple transcription factors (TFs) that bind cooperatively to cis-regulatory modules (CRMs) genes. These CRMs usually contain a variable number factor-binding sites (TFBSs) involved in related cellular and physiological processes. Chromatin immunoprecipitation followed by sequencing (ChIP-seq) has been effective detecting TFBSs nucleosome location identify potential genome-wide studies. Although several attempts were previously reported predict binding TFs at within comparing different ChIP-seq data, these have hampered excessive background, emerging as consequence experimental conditions. To understand complex regulatory circuits, it would be helpful reliable updated user-friendly tools assist identification for gene(s) interest. Results: Here we present INSECT (IN-silico SEarch Co-occurring Transcription factors), novel web server identifying gene sequences. By combining strategies, provides flexible analysis co-occurring TFBSs, applying differing search schemes restriction parameters. Availability implementation: is freely available http://bioinformatics.ibioba-mpsp-conicet.gov.ar/INSECT Contact: cperezcastro@ibioba-mpsp-conicet.gov.ar or pyankilevich@ ibioba-mpsp-conicet.gov.ar Supplementary information: data are Bioinformatics online.