Screening of differentially expressed immune-related genes from spleen of broilers fed with probiotic Bacillus cereus PAS38 based on suppression subtractive hybridization.
作者： Jiajun Li , Wanqiang Li , Jianzhen Li , Zhenhua Wang , Dan Xiao
DOI: 10.1371/JOURNAL.PONE.0226829
关键词:
摘要: The aim of this study was to construct the spleen differential genes library broilers fed with probiotic Bacillus cereus PAS38 by suppression subtractive hybridization (SSH) and screen immune-related genes. Sixty seven-day-old were randomly divided into two groups. control group basal diet, treated diet containing 1×106 CFU/g. Spleen tissues taken extracted its total RNA at 42 days old, then SSH used gene A 119 differentially expressed sequence tags (ESTs) isolated 9 screened out Gene ontology analysis. Nine identified qRT-PCR. JCHAIN, FTH1, P2RX7, TLR7, IGF1R, SMAD7, SLC7A6 found be significantly up-regulated in group. Which consistent results SSH. These findings imply that PAS38-induced might play an important role improvement immunity for broilers, which provided useful information further understanding molecular mechanism probiotics responsible affect poultry immunity.
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