Detection of Mycobacterium tuberculosis Complex in New World Monkeys in Peru.

摘要: The Mycobacterium tuberculosis complex causes in humans and nonhuman primates is a global public health concern. Standard diagnostics rely upon host immune responses to detect infection lack sensitivity specificity across the spectrum of mycobacterial these species. We have previously shown that Oral Swab PCR (OSP) assay, direct pathogen detection method, can identify presence M. laboratory free-ranging Old World monkeys. Addressing current limitations primates, including sample acquisition detection, this paper furthers our understanding tuberculosis-causing bacteria among New monkeys close contact with humans. Here we use minimally invasive OSP which includes buccal swab collection followed by amplification IS6110 repetitive nucleic acid sequence specific subspecies, mouths Peruvian A total 220 swabs from 16 species were obtained positive was observed 30 (13.6%) samples. To knowledge, first documentation DNA diverse Neotropical primates.