Optimizing a Noninvasive Oral Sampling Technique for Semicaptive Neotropical Primates in Peru.

摘要: Disease surveillance in Neotropical primates (NP) is limited by the difficulties associated with anesthetizing NP for sample collection remote settings. Our objective was to optimize a noninvasive method of oral sampling from semicaptive Peru. We offered 40 at Taricaya Rescue Centre Madre de Dios, Peru ropes coated various attractants and measured variables (acceptance rope, chewing time, volume fluid eluted ropes) that may affect acquisition quality. preserved samples direct freezing liquid nitrogen or storing RNA stabilization reagent room temperature. Sample integrity testing mammalian cytochrome b use conventional PCR. The successfully chewed on rope 82% (125/152) trials. Overall high, 96% (44/46) (both directly frozen stored reagent) positive b. number times an individual exposed procedure age were higher acceptance rates rope. conclude serve as feasible obtaining rescue centers could be used future studies evaluate population genetics pathogen health monitoring.