GENE EXPRESSION KINETICS AND PROTEIN DISTRIBUTION OF NUCLEOTIDE EXCISION REPAIR FACTORS IN THE INNER EAR AS A FUNCTION OF cis-DIAMMINEDICHLOROPLATINUM-II DNA DAMAGE

摘要: The kinetics of the rate-limiting genes molecular DNA repair pathways nucleotide excision (NER) were quantified from inner ear as a function cis-diamminedichloroplatinum-II (cisplatin) treatment. distribution post-translational products these was evaluated among neurons and sensory hair cells following cisplatin These NER factors (genes & products) are only potentiated by damage particularly sensitive to induced damage. A 2 x 3 factorial design, consisting two treatment conditions (saline treated Fischer344 rats), three survival times analysis methods (polymerase chain reaction immunohistochemistry) employed in this dissertation. results revealed at least five important findings. First, it for first time that complex such exist ear. Second, molecules used advanced tumor detect damaged genotoxicity also generalize stimulated even small sub-toxic doses cisplatin. Third, proteins reside cytoplasm under normal translocate nucleus genomic stress. Fourth, basal coil mammalian cochlea differs apical magnitude latency which Fifth, current work provides bases new line hearing research focused on mechanisms repair.