Characterization of the starch degradation activity from newly isolated Rhizopus oryzae WCS-1 and mixed cultures with Saccharomyces cerevisiae for efficient ethanol production from starch

作者: Seung-Won Jang , Jin-Seong Kim , Jae-Bum Park , Jong-Hyun Jung , Cheon-Seok Park

DOI: 10.1007/S10068-015-0235-4

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摘要: A starch degrading fungi was isolated from nuruk, a Korean traditional starter culture made with wheat flour. This newly isolated fungi was identified as Rhizopus oryzae WCS-1 via internal transcribed spacer region sequencing. Enzymatic assays for starch degradation activities indicated an optimum temperature and pH of 55° C and pH 4.0, respectively, using partially purified enzymes from R. oryzae WCS-1. Mixed cultures with S. cerevisiae CEN-PK 2-1D, L2612, BY4742, and D452-2 were performed. S. cerevisiae CEN-PK 2-1D has a …

参考文章(29)
Zhizhuang Xiao, Meiqun Wu, Manon Beauchemin, Denis Groleau, Peter C.K. Lau, ORIGINAL RESEARCH: Direct fermentation oftriticale starch to lactic acid by Rhizopus oryzae Industrial Biotechnology. ,vol. 7, pp. 129- 134 ,(2011) , 10.1089/IND.2011.7.129
Barnita Ghosh, Rina Rani Ray, Characterization of Raw Starch Digesting and Adsorbing Extra Cellular Isoamylase from Rhizopus oryzae Recent Research in Science and Technology. ,vol. 2, pp. 64- 70 ,(2010)
Chen-Hsi Chu, Kun-Mou Li, Shih-Wei Lin, Margaret Dah-Tsyr Chang, Ting-Ying Jiang, Yuh-Ju Sun, Crystal structures of starch binding domain from Rhizopus oryzae glucoamylase in complex with isomaltooligosaccharide: insights into polysaccharide binding mechanism of CBM21 family. Proteins. ,vol. 82, pp. 1079- 1085 ,(2014) , 10.1002/PROT.24446
Hisayori Shigechi, Jun Koh, Yasuya Fujita, Takeshi Matsumoto, Yohei Bito, Mitsuyoshi Ueda, Eiichi Satoh, Hideki Fukuda, Akihiko Kondo, Direct Production of Ethanol from Raw Corn Starch via Fermentation by Use of a Novel Surface-Engineered Yeast Strain Codisplaying Glucoamylase and α-Amylase Applied and Environmental Microbiology. ,vol. 70, pp. 5037- 5040 ,(2004) , 10.1128/AEM.70.8.5037-5040.2004
PC Ashly, PV Mohanan, None, Preparation and characterization of Rhizopus amyloglucosidase immobilized on poly(o-toluidine) Process Biochemistry. ,vol. 45, pp. 1422- 1426 ,(2010) , 10.1016/J.PROCBIO.2010.04.022
Haq Nawaz Bhatti, Mohammad Hamid Rashid, Rakhshanda Nawaz, Muhammad Asgher, Raheela Perveen, Abdul Jabbar, Purification and characterization of a novel glucoamylase from Fusarium solani Food Chemistry. ,vol. 103, pp. 338- 343 ,(2007) , 10.1016/J.FOODCHEM.2006.07.058
Aloia Romaní, Remedios Yáñez, Gil Garrote, José Luis Alonso, SSF production of lactic acid from cellulosic biosludges. Bioresource Technology. ,vol. 99, pp. 4247- 4254 ,(2008) , 10.1016/J.BIORTECH.2007.08.051
Óscar J. Sánchez, Carlos A. Cardona, Trends in biotechnological production of fuel ethanol from different feedstocks. Bioresource Technology. ,vol. 99, pp. 5270- 5295 ,(2008) , 10.1016/J.BIORTECH.2007.11.013
Kyohei Mizokami, Hiroaki Katsura, Yoshinori Okita, Shinya Sekitou, Hideo Takahashi, Takehiko Yamamoto, Shifts in the Optimum pH of Rhizopus Glucoamylase Depending on the Reaction Temperatures. Bioscience, Biotechnology, and Biochemistry. ,vol. 58, pp. 183- 184 ,(1994) , 10.1271/BBB.58.183