Centrosome aberration accompanied with p53 mutation can induce genetic instability in hepatocellular carcinoma.

摘要: Centrosome duplication is controlled in a cell cycle-specific manner and occurs once every cycle, thereby ensuring the balanced segregation of chromosomes during mitotic phase. Numerical or structural abnormalities can arise centrosomes malignant cells. Under defective cycle checkpoint systems, cancer cells with abnormal survive re-enter promoting unbalanced chromosome genetic instability. We investigated centrosome aberrations 33 patients diagnosed hepatocellular carcinoma (HCC), using fluorescent pericentrin immunostaining. also studied p53 mutation, proliferative activity, DNA ploidy these cases. In normal hepatocytes, one was identified per as round dot, usually vicinity nuclear membrane. However, from HCC tissue, several patterns occurred, including supernumerary an shape size. Although frequency each tissue relatively low compared previous reports other cancers, nevertheless, aberration found 30 out tissues. The percentage tumor significantly higher nondiploid tumors (15.8±15.9‰) than diploid (5.4±5.1‰) (P<0.05), tended to be mutation (11.6±13.1‰) those wild-type (5.6±6.8‰). Furthermore, 82% exhibited whereas only 41% showed mutation. were not related stage, size activity. Therefore, our results indicate that hepatic cells, under system possibly caused by have potential for instability aggressive behavior. This effect irrespective stage.