Procyclic tsetse fly midgut forms and culture forms of African trypanosomes share stage- and species-specific surface antigens identified by monoclonal antibodies.

摘要: Procyclic culture form (PCF) trypanosomes were established from a bloodstream population of cloned Trypanosoma brucei rhodesiense and used to immunize mice for hybridoma production. Indirect immunofluorescence was select 10 hybridomas which secreted antibodies that bound the surface homologous living PCF. The reacted with PCF several clones T.b. brucei, gambiense, rhodesiense, but not T. congolense or vivax, promastigotes species Leishmania parasites. antigens detectable in ethanol/acetic acid-fixed forms lysates any subspecies, are thus species-specific stage-specific markers. Selected monoclonal procyclic taken directly midgut infected tsetse flies, immature epimastigote salivary probes, may therefore be useful epidemiologic investigations.