Soluble expression and sodium channel activity of lt16a, a novel framework XVI conotoxin from the M-superfamily.
作者： Maojun Zhou , Lei Wang , Yun Wu , Junliang Liu , Dandan Sun
DOI: 10.1016/J.TOXICON.2015.01.009
关键词:
摘要: A peptide toxin, lt16a, from the venom of worm-hunting Conus litteratus, shares typical signal sequences M-superfamily conotoxins, which usually contain six cysteine residues that are arranged in a CC-C-C-CC pattern. Interestingly, lt16a comprises 21 amino acid its mature region and has framework XVI, is C-C-CC The coding was cloned into pTRX vector fusion protein overexpressed Escherichia coli. After cleaving purifying using chromatography, mass found by spectrometry to be consistent with expected 2357.7 Da. Whole-cell patch clamp experiments demonstrated could inhibit both TTX-sensitive TTX-resistant sodium currents adult rat dorsal root ganglion neurons. inhibition on stronger than currents. To our knowledge, this first report XVI conotoxin can voltage-gated channel mammalian sensory This helps facilitates an understanding sequence diversity conotoxins.
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