Evaluation of the relatedness of strains of Mycobacterium avium using pulsed-field gel electrophoresis

摘要: The application of molecular techniques to investigate strain relatedness may help define the local epidemiology ofMycobacterium avium infection, and, by identifying false isolates (i.e. neither pathogens nor colonizers) resulting from contamination, serve as a tool for quality control in laboratory. For this purpose, all patients (n=129) withMycobacterium infections identified over two-year period were investigated pulsed-field gel electrophoresis (PFGE). Of 38 PFGE patterns identified, 34 corresponded unique strains or present no more than two three individuals. One prevalent was among HIV-infected and related culture contamination events. (i) established diversity community; (ii) existence that account one-fifth isolated locally; (iii) documented extent resolution suspected pseudo-outbreak; (iv) uncovered an additional unsuspected event.