Rapid identification of mycobacteria to the species level by polymerase chain reaction and restriction enzyme analysis.

摘要: A method for the rapid identification of mycobacteria to species level was developed on basis evaluation by polymerase chain reaction (PCR) gene encoding 65-kDa protein. The involves restriction enzyme analysis PCR products obtained with primers common all mycobacteria. Using two enzymes, BstEII and HaeIII, medically relevant other frequent laboratory isolates were differentiated or subspecies PCR-restriction pattern analysis. performed (n = 330) from solid fluid culture media, including BACTEC, frozen lyophilized stocks. procedure does not involve hybridization steps use radioactivity can be completed within 1 working day. Images