Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

摘要: CRISPR/Cas9-mediated genome editing is a next-generation strategy for genetic modifications, not only single gene targeting, but also multiple targeted mutagenesis. To make the most of multiplexity CRISPR/Cas9, we established system constructing all-in-one expression vectors containing guide RNA cassettes and Cas9 nuclease/nickase cassette. We further demonstrated successful examples targeting including chromosomal deletions in human cells using CRISPR/Cas9 constructed with our novel system. Our provides an efficient multiplex genome/epigenome editing, simultaneous activation/repression genes, beyond.