Custom microarray for glycobiologists: considerations for glycosyltransferase gene expression profiling.

摘要: The development of microarray technology offers the unprecedented possibility studying expression thousands genes in one experiment. Its exploitation glycobiology field will eventually allow parallel investigation many glycosyltransferases, which ultimately lead to an understanding regulation glycoconjugate synthesis. While numerous gene arrays are available on market, e.g. Affymetrix GeneChip® arrays, glycosyltransferases not adequately represented, makes comprehensive surveys their difficult. This chapter describes main issues related establishment a custom glycogenes array.