Nucleotide preferences in sequence-specific recognition of DNA by c-myb protein

摘要: Using a binding site selection procedure, we have found that sequence-specific DNA-binding by the mouse c-myb protein involves recognition of nucleotides outside previously identified hexanucleotide motif. Oligonucleotides containing random nucleotide core were immunoprecipitated in association with c-Myb, amplified Polymerase Chain Reaction and cloned plasmids prior to sequencing. By alignment sequences it was apparent additional preferences existed at each three bases immediately 5' consensus, allowing an extension preferred YGRCVGTTR. The contributions these affinity established bandshift analyses oligonucleotides single base substitutions; particular, replacement guanine position -2 any other greatly reduced c-Myb binding. We encoded related B-myb gene bound similar affinity; however, B-Myb showed distinct for identity -1 relative consensus. This study demonstrates is more extensive than recognised hitherto points but DNA Myb proteins.