Knockdown of DGCR5 enhances the radiosensitivity of human laryngeal carcinoma cells via inducing miR‐195
作者： Tian Tang , Guang Shan , Feng Zeng
DOI: 10.1002/JCP.27958
关键词:
摘要: Long noncoding RNAs (lncRNAs) exert critical roles in the development of various cancers, including human laryngeal cancer. Radioresistance contributes to predominant causes cancer recurrence after radiotherapy. The aim our study was investigate association dysregulated lncRNA and radiation resistance larynx squamous carcinoma. Here, we investigated biological DiGeorge syndrome region gene 5 (DGCR5) radioresistance Two carcinoma cell lines (Hep-2 Hep-2R), with different radiosensitivities vitro were used present study. We observed that DGCR5 significantly upregulated Hep-2R cells. Inhibition by LV-shDGCR5 transfection restrained proliferation sensitized cells radiation. Reversely, overexpression exhibited an opposite phenomenon vitro. In addition, microRNA (miR)-195 predicted as a direct downstream target DGCR5. Dual-luciferase reporter RNA immunoprecipitation assays verified interaction between them. Meanwhile, miR-195 be reduced mimics repressed Hep-2 growth. Moreover, radiosensitivity greatly enhanced miR-195, which could reversed upregulation Finally, vivo experiments validate knockdown suppressed via targeting miR-195. conclusion, indicated contribute sponging
sci-hub.se 参考文章(30)
Aymen Lagha, Nesrine Chraiet, Soumaya Labidi, Hela Rifi, Mouna Ayadi, Sarra Krimi, Bassem Allani, Henda Raies, Slim Touati, Hamouda Boussen, Lotfi Kochbati, Amel Mezlini, Larynx preservation: What is the best non-surgical strategy? Critical Reviews in Oncology Hematology. ,vol. 88, pp. 447- 458 ,(2013) , 10.1016/J.CRITREVONC.2013.05.005
Joshua T. Mendell, Eric N. Olson, MicroRNAs in Stress Signaling and Human Disease Cell. ,vol. 148, pp. 1172- 1187 ,(2012) , 10.1016/J.CELL.2012.02.005
Matthew K Iyer, Yashar S Niknafs, Rohit Malik, Udit Singhal, Anirban Sahu, Yasuyuki Hosono, Terrence R Barrette, John R Prensner, Joseph R Evans, Shuang Zhao, Anton Poliakov, Xuhong Cao, Saravana M Dhanasekaran, Yi-Mi Wu, Dan R Robinson, David G Beer, Felix Y Feng, Hariharan K Iyer, Arul M Chinnaiyan, The landscape of long noncoding RNAs in the human transcriptome Nature Genetics. ,vol. 47, pp. 199- 208 ,(2015) , 10.1038/NG.3192
Nijiro Nohata, Toyoyuki Hanazawa, Takashi Kinoshita, Yoshitaka Okamoto, Naohiko Seki, MicroRNAs function as tumor suppressors or oncogenes: Aberrant expression of microRNAs in head and neck squamous cell carcinoma Auris Nasus Larynx. ,vol. 40, pp. 143- 149 ,(2013) , 10.1016/J.ANL.2012.07.001
Guodong Yang, Xiaozhao Lu, Lijun Yuan, LncRNA: a link between RNA and cancer. Biochimica et Biophysica Acta. ,vol. 1839, pp. 1097- 1109 ,(2014) , 10.1016/J.BBAGRM.2014.08.012
Rory Johnson, Long non-coding RNAs in Huntington's disease neurodegeneration. Neurobiology of Disease. ,vol. 46, pp. 245- 254 ,(2012) , 10.1016/J.NBD.2011.12.006
Hassan Arshad, Vijayvel Jayaprakash, Vishal Gupta, David M. Cohan, Dildeep Ambujakshan, Nestor R. Rigual, Anurag K. Singh, Wesley L. Hicks, Survival Differences between Organ Preservation Surgery and Definitive Radiotherapy in Early Supraglottic Squamous Cell Carcinoma Otolaryngology-Head and Neck Surgery. ,vol. 150, pp. 237- 244 ,(2014) , 10.1177/0194599813512783
Ahmedin Jemal, Freddie Bray, Melissa M. Center, Jacques Ferlay, Elizabeth Ward, David Forman, Global cancer statistics CA: A Cancer Journal for Clinicians. ,vol. 61, pp. 69- 90 ,(1999) , 10.3322/CAAC.20107
Zhisen Shen, Qun Li, Hongxia Deng, Dakai Lu, Haojun Song, Junming Guo, Long Non-Coding RNA Profiling in Laryngeal Squamous Cell Carcinoma and Its Clinical Significance: Potential Biomarkers for LSCC PLoS ONE. ,vol. 9, pp. e108237- ,(2014) , 10.1371/JOURNAL.PONE.0108237
Yu Jinjin, Shen Zongji, Wang Yuan, Han Xiaoyun, Qiu Haifeng, Li Jing, Hu Weixu, Dong Ruofan, MicroRNA-218 Enhances the Radiosensitivity of Human Cervical Cancer via Promoting Radiation Induced Apoptosis International Journal of Medical Sciences. ,vol. 11, pp. 691- 696 ,(2014) , 10.7150/IJMS.8880