Is Nor region variability in wheat invariably caused by tissue culture
作者： A. Breiman , T. Felsenburg , E. Galun
DOI: 10.1007/BF00268331
关键词:
摘要: In a previous study we observed extensive Nor region variability in tissue-culture derived plants of only one out three tested wheat cultivars. This finding prompted us to further question whether or not this was invariably caused by vitro culture. the present study, upper halves spikes from four source inbred cultivar ‘ND7532’ were removed 12 days after anthesis. The immature embryos these cultured and regenerated into plants. lower same retained on obtain mature caryopses. DNA extracted seedlings, cut with TaqI endonuclease, run agarose gels, respective Southern blots probed plasmid pTA71 reveal patterns. sexual progeny regenerants provided patterns which exactly identical obtained seedlings germinated caryopses matured spikes. fourth plant variable Analyses 21 individual showed that 18 had three-fragment pattern (consisting 3.0, 2.7 1.9 kb fragments) while lacked (2.7 kb) fragment. Furthermore, next segregated three- two-fragment patterns.These results, conjunction reports among plants, suggest is related
springer.com
sci-hub.se 参考文章(21)
Jan Dvořák, R Appels, Chromosome and nucleotide sequence differentiation in genomes of polyploid Triticum species. Theoretical and Applied Genetics. ,vol. 63, pp. 349- 360 ,(1982) , 10.1007/BF00303906
D. B. Cooper, R. G. Sears, G. L. Lookhart, B. L. Jones, Heritable somaclonal variation in gliadin proteins of wheat plants derived from immature embryo callus culture. Theoretical and Applied Genetics. ,vol. 71, pp. 784- 790 ,(1986) , 10.1007/BF00276418
W.L. Gerlach, J.R. Bedbrook, Cloning and characterization of ribosomal RNA genes from wheat and barley Nucleic Acids Research. ,vol. 7, pp. 1869- 1885 ,(1979) , 10.1093/NAR/7.7.1869
A. Benslimane, C. Hartmann, J. de Buyse, Y. Henry, E. Picard, A. Rode, Ribosomal DNA as a convenient probe to follow segregation and possible divergency from expected homozygosity after haploidization of an androgenetic process Theoretical and Applied Genetics. ,vol. 75, pp. 389- 396 ,(1988) , 10.1007/BF00276740
S. S. Virmani, R. C. Aquino, G. S. Khush, Heterosis breeding in rice (Oryza sativa L.). Theoretical and Applied Genetics. ,vol. 63, pp. 373- 380 ,(1982) , 10.1007/BF00303911
S. A. Ryan, W. R. Scowcroft, A somaclonal variant of wheat with additional β-amylase isozymes. Theoretical and Applied Genetics. ,vol. 73, pp. 459- 464 ,(1987) , 10.1007/BF00262516
E.M. Southern, Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology. ,vol. 98, pp. 503- 517 ,(1975) , 10.1016/S0022-2836(75)80083-0
Gisela Gosch-Wackerle, Lydia Avivi, Esra Galun, Induction, Culture and Differentiation of Callus from Immature Rachises, Seeds and Embryos of Triticum Zeitschrift für Pflanzenphysiologie. ,vol. 91, pp. 267- 278 ,(1979) , 10.1016/S0044-328X(79)80100-2
J. Landsmann, H. Uhrig, Somaclonal variation in Solanum tuberosum detected at the molecular level Theoretical and Applied Genetics. ,vol. 71, pp. 500- 505 ,(1985) , 10.1007/BF00251196
Variation in the Intergenic Spacer of Ribosomal DNA of Wild Wheat, Triticum dicoccoides. In Israel Molecular Biology and Evolution. ,vol. 3, pp. 547- 547 ,(1986) , 10.1093/OXFORDJOURNALS.MOLBEV.A040418