Identification of metabolites of liquiritin in rats by UHPLC-Q-TOF-MS/MS: metabolic profiling and pathway comparison in vitro and in vivo
作者： Xia Zhang , Caijuan Liang , Jintuo Yin , Yupeng Sun , Lantong Zhang
DOI: 10.1039/C7RA13760E
关键词:
摘要: Liquiritin (LQ), the main bioactive constituent of licorice, is a common flavoring and sweetening agent in food products has wide range pharmacological properties, including antidepressant-like, neuroprotective, anti-cancer anti-inflammatory properties. This study investigated metabolic pathways LQ vitro (rat liver microsomes) vivo model) using ultra high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). Moreover, supplementary tools such as key product ions (KPIs) were employed to search for identify compounds. As result, 56 metabolites 15 structurally characterized. Oxidation, reduction, hydrolysis, methylation, acetylation, sulfate glucuronide conjugation determined be major LQ, there differences metabolism vivo. In addition, compared this study.
rsc.org
sci-hub.se 参考文章(46)
Jianmei Wang, Zhen Yang, James Lechago, Rapid and simultaneous determination of multiple classes of abused drugs and metabolites in human urine by a robust LC-MS/MS method – application to urine drug testing in pain clinics Biomedical Chromatography. ,vol. 27, pp. 1463- 1480 ,(2013) , 10.1002/BMC.2945
Han Han, Wenliang Zeng, Chunyong He, S. W. Annie Bligh, Qing Liu, Li Yang, Zhengtao Wang, Characterization of metabolites of sweroside in rat urine using ultra-high-performance liquid chromatography combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry and NMR spectroscopy. Journal of Mass Spectrometry. ,vol. 49, pp. 1108- 1116 ,(2014) , 10.1002/JMS.3429
Xiangyu Meng, Shibin Yang, Zifeng Pi, Fengrui Song, Hongyu Jiang, Zhiqiang Liu, AN INVESTIGATION OF THE METABOLISM OF LIQUIRITIN AND THE IMMUNOLOGICAL EFFECTS OF ITS METABOLITES Journal of Liquid Chromatography & Related Technologies. ,vol. 35, pp. 1538- 1549 ,(2012) , 10.1080/10826076.2011.619042
Guangguo Tan, Min Liu, Xin Dong, Si Wu, Li Fan, Youbei Qiao, Yifeng Chai, Hong Wu, A strategy for rapid analysis of xenobiotic metabolome of Sini decoction in vivo using ultra-performance liquid chromatography-electrospray ionization quadrupole-time-of-flight mass spectrometry combined with pattern recognition approach Journal of Pharmaceutical and Biomedical Analysis. ,vol. 96, pp. 187- 196 ,(2014) , 10.1016/J.JPBA.2014.03.028
James P. Scarth, Holly A. Spencer, Sarah E. Timbers, Simon C. Hudson, Lynn L. Hillyer, The use of in vitro technologies coupled with high resolution accurate mass LC-MS for studying drug metabolism in equine drug surveillance. Drug Testing and Analysis. ,vol. 2, pp. 1- 10 ,(2010) , 10.1002/DTA.88
G. Khaksa, M. Zolfaghari, A. Dehpour, T. Samadian, Anti-Inflammatory and Anti-Nociceptive Activity of Disodium Glycyrrhetinic Acid Hemiphthalate Planta Medica. ,vol. 62, pp. 326- 328 ,(1996) , 10.1055/S-2006-957894
Jing Liang, Feng Xu, Ya-Zhou Zhang, Shuai Huang, Xin-Yu Zang, Xin Zhao, Lei Zhang, Ming-Ying Shang, Dong-Hui Yang, Xuan Wang, Shao-Qing Cai, The profiling and identification of the absorbed constituents and metabolites of Paeoniae Radix Rubra decoction in rat plasma and urine by the HPLC–DAD–ESI-IT-TOF-MSn technique: A novel strategy for the systematic screening and identification of absorbed constituents and metabolites from traditional Chinese medicines Journal of Pharmaceutical and Biomedical Analysis. ,vol. 83, pp. 108- 121 ,(2013) , 10.1016/J.JPBA.2013.04.029
Ya-Zhou Zhang, Feng Xu, Jing Dong, Jing Liang, Yuki Hashi, Ming-Ying Shang, Dong-Hui Yang, Xuan Wang, Shao-Qing Cai, Profiling and identification of the metabolites of calycosin in rat hepatic 9000 × g supernatant incubation system and the metabolites of calycosin-7-O-β-d-glucoside in rat urine by HPLC–DAD–ESI-IT-TOF-MSn technique Journal of Pharmaceutical and Biomedical Analysis. ,vol. 70, pp. 425- 439 ,(2012) , 10.1016/J.JPBA.2012.06.006
Alan Hb Wu, Roy Gerona, Patil Armenian, Deborah French, Matthew Petrie, Kara L Lynch, Role of liquid chromatography-high-resolution mass spectrometry (LC-HR/MS) in clinical toxicology. Clinical Toxicology. ,vol. 50, pp. 733- 742 ,(2012) , 10.3109/15563650.2012.713108
Iwona Wilk-Zasadna, Camilla Bernasconi, Olavi Pelkonen, Sandra Coecke, Biotransformation in vitro: An essential consideration in the quantitative in vitro-to-in vivo extrapolation (QIVIVE) of toxicity data. Toxicology. ,vol. 332, pp. 8- 19 ,(2015) , 10.1016/J.TOX.2014.10.006