A time-of-flight mass spectrometry based strategy to fast screen triterpenoids in Xanthoceras sorbifolia Bunge husks for bioactive substances against Alzheimer's disease

摘要: Xanthoceras sorbifolia Bunge is a folk medicine in China. Recently, the triterpenoids its husks have attracted more and attention for potential prevention against Alzheimer's disease. However, current studies on bioactive substances were still insufficient. To reveal substances, an efficient practical strategy based high resolution mass spectra coupled with multiple data mining techniques was developed to characterize barrigenol type dosed rat plasma. A total of 50 identified husks, 6 these detected plasma, which regarded as candidates. find real neuroprotective effect candidates further tested by calculating PC12 cell viability amyloid-β-induced cytotoxicity. As result, three out six exhibited obvious neuroprotction cytotoxicity cells, indicating their be This study will valuable reference disease provided can also applied exploration effective constituents other medicines.