A protein induced by NGF in PC12 cells is stored in secretory vesicles and released through the regulated pathway.

摘要: We have previously described the isolation of a cDNA clone corresponding to an mRNA rapidly induced high levels in PC12 cells by treatment with NGF. report here complete amino acid sequence protein (named VGF8a) as deduced nucleotide sequencing overlapping clones. VGF8a is particularly rich proline residues and has conspicuous number short stretches basic which may represent potential targets for proteolytic cleavage. Antibodies directed against recombinant VGF8a-beta-galactosidase fusion proteins were used immunofluorescent staining well its localization, Western blot analysis, subfractions cell homogenates. demonstrate that cells, stored secretory vesicles released response variety stimuli are known induce regulated secretion neurotransmitters.