High-Throughput Phenotyping of Root Growth Dynamics

摘要: Plant organ phenotyping by noninvasive video imaging techniques provides a powerful tool to assess physiological traits, circadian and diurnal rhythms, biomass production. In particular, growth of individual plant organs is known exhibit high plasticity occurs as result the interaction between various endogenous environmental processes. Thus, any investigation aiming unravel mechanisms that determine or has accurately control document conditions. Here we describe challenges in establishing recently developed root monitoring platform (PlaRoM) specially suited for high-throughput analysis with highest emphasis on detailed documentation capture time, well light temperature Furthermore, discuss experimental procedure measuring elongation kinetics key points must be considered such measurements. PlaRoM consists robotized enclosed custom designed phytochamber extension profiling software application. This been multi-parallel recordings phenotypes up 50 seedlings over several days, spatial temporal resolution. Two Petri dishes are mounted vertical sample stage exact control. A computer-controlled positioning unit moves these small increments enables continuous screening surface under binocular microscope. Detection tip achieved applying thresholds image pixel data verifying neighbourhood each dark pixel. The parameters visualized position time rate graphs averaged consecutive light-dark periods 24 h day periods. setup profiles different genotypes conditions (e.g., protocol, temperature, media) especially detection rhythms.