Suppressing the growth of rectal cancer xenografts derived from patient tumors by an adenovector expressing small hairpin RNA targeting Bcl-XL.
作者： Hongbo Zhu , Wei Zhou , Jingzi Hu , Zhongting Huang , Weifeng Lao
DOI: 10.1002/JGM.2681
关键词:
摘要: Background Bcl-XL, a mitochondria membrane protein, is overexpressed in colorectal cancers and promotes cell survival. We have previously shown that the adenovector expressing small hairpin (sh)RNA targeting Bcl-XL could induce significantly apoptosis colon cancer cells. In present study, we aimed to further detect anti-cancer effect of shRNA (Ad/Bcl-XL shRNA) on rectal xenografts were derived from patient tumors. Methods We first established three xenografts. These subsequently treated with Ad/Bcl-XL alone or combination 5-fluouracil (5-Fu). Finally, inhibition tumor growth, survival time induction analyzed. Results The results obtained demonstrated effectively suppress growth all prolong their time. After being combined 5-Fu, suppressing was enhanced further. addition, data also showed 5-Fu increase apoptotic ratio xenograft. Conclusions These indicate without has effective anti-tumor effects tumor-derived xenografts, suggesting it be potential strategy for therapy. Copyright © 2012 John Wiley & Sons, Ltd.
sci-hub.se 参考文章(24)
K. Smid, P. S. Schoenmakers, G. Jansen, C. L. Van Der Wilt, B. Van Triest, F. Telleman, P. Noordhuis, J. A. M. Van Laar, Y. Van Hensbergen, H. M. Pinedo, G. J. Peters, Thymidylate synthase level as the main predictive parameter for sensitivity to 5-fluorouracil, but not for folate-based thymidylate synthase inhibitors, in 13 nonselected colon cancer cell lines. Clinical Cancer Research. ,vol. 5, pp. 643- 654 ,(1999)
Rebecca Liu, Carmen Page, David R. Beidler, Max S. Wicha, Gabriel Núñez, Overexpression of Bcl-xL Promotes Chemotherapy Resistance of Mammary Tumors in a Syngeneic Mouse Model American Journal of Pathology. ,vol. 155, pp. 1861- 1867 ,(1999) , 10.1016/S0002-9440(10)65505-8
W Zhou, H Zhu, W Chen, X Hu, X Pang, J Zhang, X Huang, B Fang, C He, Treatment of patient tumor-derived colon cancer xenografts by a TRAIL gene-armed oncolytic adenovirus Cancer Gene Therapy. ,vol. 18, pp. 336- 345 ,(2011) , 10.1038/CGT.2010.83
Gayle C. Bosma, R. Philip Custer, Melvin J. Bosma, A severe combined immunodeficiency mutation in the mouse Nature. ,vol. 301, pp. 527- 530 ,(1983) , 10.1038/301527A0
Xin Shi, Shengli Liu, Jörg Kleeff, Helmut Friess, Markus W. Büchler, Acquired resistance of pancreatic cancer cells towards 5-Fluorouracil and gemcitabine is associated with altered expression of apoptosis-regulating genes. Oncology. ,vol. 62, pp. 354- 362 ,(2002) , 10.1159/000065068
Christopher L Morton, Peter J Houghton, Establishment of human tumor xenografts in immunodeficient mice. Nature Protocols. ,vol. 2, pp. 247- 250 ,(2007) , 10.1038/NPROT.2007.25
I. Fichtner, W. Slisow, J. Gill, M. Becker, B. Elbe, T. Hillebrand, M. Bibby, Anticancer drug response and expression of molecular markers in early-passage xenotransplanted colon carcinomas. European Journal of Cancer. ,vol. 40, pp. 298- 307 ,(2004) , 10.1016/J.EJCA.2003.10.011
K Benihoud, S Esselin, D Descamps, B Jullienne, B Salone, P Bobé, D Bonardelle, E Connault, P Opolon, I Saggio, M Perricaudet, Respective roles of TNF-alpha and IL-6 in the immune response-elicited by adenovirus-mediated gene transfer in mice. Gene Therapy. ,vol. 14, pp. 533- 544 ,(2007) , 10.1038/SJ.GT.3302885
Hongbo Zhu, Wei Gup, Lidong Zhang, John J. Davis, Shuhong Wu, Fuminori Teraishi, Xiaobo Cao, W. Roy Smythe, Bingliang Fang, Enhancing TRAIL-induced apoptosis by Bcl-XL siRNA Cancer Biology & Therapy. ,vol. 4, pp. 393- 397 ,(2005) , 10.4161/CBT.4.4.1616
Ketao Jin, Lisong Teng, Yanping Shen, Kuifeng He, Zhenzhen Xu, Guangliang Li, Patient-derived human tumour tissue xenografts in immunodeficient mice: a systematic review Clinical and Translational Oncology. ,vol. 12, pp. 473- 480 ,(2010) , 10.1007/S12094-010-0540-6