In vitro storage under slow growth and ex vitro acclimatization of the ornamental bromeliad Acanthostachys strobilacea

作者: Victória de Carvalho , Daniela Soares dos Santos , Catarina Carvalho Nievola

DOI: 10.1016/J.SAJB.2014.01.011

关键词:

摘要: Abstract An in vitro short-term storage method for Acanthostachys strobilacea (Schult. f.) Link, Klotzsch & Otto plants and subsequent ex acclimatization were examined. Seedlings cultured at 20, 15, 10 °C (low temperatures) 25 °C (control) 30, 60, 90 days. After each period, the transferred to trays containing Pinus bark substrate maintained 60 days. 90 days of culture, lowest biometric parameters mean values found grown (approximately 4 times smaller than control). These successfully acclimatized with 100% survival rapid regrowth, while they exhibited a similar phenotype chlorophyll content control treatment. The results showed that is appropriate A. when cultivated vitro.

参考文章(21)
Toyoki Kozai, Chieri Kubota, Byoung Ryoung Jeong, Environmental control for the large-scale production of plants through in vitro techniques Plant Cell Tissue and Organ Culture. ,vol. 51, pp. 49- 56 ,(1997) , 10.1023/A:1005809518371
Hartmut K. Lichtenthaler, [34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes Methods in Enzymology. ,vol. 148, pp. 350- 382 ,(1987) , 10.1016/0076-6879(87)48036-1
Grazia Marino, Paola Negri, Antonio Cellini, Andrea Masia, Effect of carbohydrates on in vitro low-temperature storage of shoot cultures of apricot Scientia Horticulturae. ,vol. 126, pp. 434- 440 ,(2010) , 10.1016/J.SCIENTA.2010.08.008
Daniela Soares dos Santos, Vívian Tamaki, Catarina Carvalho Nievola, In vitro propagation of the ornamental bromeliad Acanthostachys strobilacea (Schult. f.) Klotzsch via nodal segments. In Vitro Cellular & Developmental Biology – Plant. ,vol. 46, pp. 524- 529 ,(2010) , 10.1007/S11627-010-9325-4
Camila Pereira Carvalho, Adriana Hissae Hayashi, Marcia Regina Braga, Catarina Carvalho Nievola, Biochemical and anatomical responses related to the in vitro survival of the tropical bromeliad Nidularium minutum to low temperatures Plant Physiology and Biochemistry. ,vol. 71, pp. 144- 154 ,(2013) , 10.1016/J.PLAPHY.2013.07.005
Toshio Murashige, Folke Skoog, A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures Physiologia Plantarum. ,vol. 15, pp. 473- 497 ,(1962) , 10.1111/J.1399-3054.1962.TB08052.X
Teresa Orlikowska, Marta Zawadzka, Danuta Kucharska, Lesław B. Lahuta, The influence of the cooling of donor cultures on the in vitro adventitious regeneration and carbohydrate metabolism of four dwarfing apple rootstocks Acta Physiologiae Plantarum. ,vol. 32, pp. 333- 340 ,(2010) , 10.1007/S11738-009-0411-3
Sonia Malik, Ravi Kumar, Surender K. Vats, Shashi Bhushan, Madhu Sharma, Paramvir Singh Ahuja, Regeneration in Rheum emodi Wall : A step towards conservation of an endangered medicinal plant species Engineering in Life Sciences. ,vol. 9, pp. 130- 134 ,(2009) , 10.1002/ELSC.200800071
Hülya Akdemir, Veysel Süzerer, Engin Tilkat, Hakan Yildirim, Ahmet Onay, Yelda Ozden Çiftçi, In vitro conservation and cryopreservation of mature pistachio (Pistacia vera L.) germplasm Journal of Plant Biochemistry and Biotechnology. ,vol. 22, pp. 43- 51 ,(2013) , 10.1007/S13562-012-0109-2
Leif Schulman, Susanna Lehvävirta, Botanic gardens in the age of climate change Biodiversity and Conservation. ,vol. 20, pp. 217- 220 ,(2011) , 10.1007/S10531-010-9979-6