作者: Victória de Carvalho , Daniela Soares dos Santos , Catarina Carvalho Nievola
DOI: 10.1016/J.SAJB.2014.01.011
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摘要: Abstract An in vitro short-term storage method for Acanthostachys strobilacea (Schult. f.) Link, Klotzsch & Otto plants and subsequent ex acclimatization were examined. Seedlings cultured at 20, 15, 10 °C (low temperatures) 25 °C (control) 30, 60, 90 days. After each period, the transferred to trays containing Pinus bark substrate maintained 60 days. 90 days of culture, lowest biometric parameters mean values found grown (approximately 4 times smaller than control). These successfully acclimatized with 100% survival rapid regrowth, while they exhibited a similar phenotype chlorophyll content control treatment. The results showed that is appropriate A. when cultivated vitro.